In recent weeks, a bioassay has been performed with Fusarium isolates collected during Uireka-1 (2017-2019). The test was performed on whole onions in accordance with a test as previously described in an English study (Tayler et al., 2016). The test showed very clearly which of the collected Fusarium oxysporum en Fusarium solani isolates are pathogenic (pathogenic).
The pathogenic and non-pathogenic isolates are now further used for whole genome sequencing (loosely translated: imaging the DNA structure). Then a search can be made for the genetic information that makes the strains pathogenic for onions or not. The next step is to develop detection methods that distinguish between onion pathogens and non-pathogens.
Collect new monsters
Although Wageningen University & Research is closed to visitors and students, work is still possible. The laboratories are open and the rules from the RIVM are strictly observed. The first soil samples have now been taken at various research locations. This is in several onion rotations prior to cultivation. This is repeated during cultivation and at the end of cultivation.
The recently taken samples were stored cold for later use for testing the detection methods. They are also used to monitor data on the increase or decrease of pathogens in the soil.
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